Lunchseminar with Johannes Björnerås from Octapharma AB


Welcome to MedTech West for a lunchseminar with Johannes Björnerås from Octapharma AB on Friday 7 September. The title of the seminar is “Dynorphin A, biophysical studies of peptide–receptor and peptide–membrane interactions”.

When? 11:30-12:30 (13:00 incl. light lunch) on Friday 7 September, 2018
Where? MedTech West conference room, Röda stråket 10B, Göteborg

NOTE! We are serving a light lunch to participants registered at the latest on Wednesday 5 September at 11:00. You are welcome to register even after that, but then we will not be able to order a lunch for you.

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Abstract:
Research concerning the opioid peptide dynorphin A (DynA) will be presented. DynA functions primarily as a neurotransmitter and belongs to the family of typical opioid peptides, part of the opioid system. This system is involved or implicated in several physiological processes such as analgesia, addiction, depression and other types of neurological disorders. Here will be discussed the interactions between DynA and the kappa-opioid receptor (KOR), as well as some aspects of DynA–membrane interactions and a small study of bicelles, a membrane mimetic.

The peptide–receptor studies were focused on the selectivity-modulating second extracellular loop (EL2) of KOR. A protein engineering approach was used in which the EL2 was grafted onto a soluble protein scaffold. Results on the interactions between DynA and EL2 in the construct protein environment will be presented.

With bicelles as a mimetic, membrane interactions were probed for wild-type DynA and for two DynA peptide variants linked to a neurological disorder. The effect of the lipid environment on peptide dynamics and structure for these DynA variants will be discussed.

Additionally, in a methodological project, DHPC/DMPC bicelle morphology as a function of total PC concentration was characterised by diffusion NMR in combination with two-way decomposition. The results will be presented, and discussed in the context of choosing when to use bicelles as a membrane mimetic.

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